Premium
Role of matrix metalloproteinase‐9 in in vitro invasion of esophageal carcinoma cells
Author(s) -
Suzuki Tomotaka,
Kuwabara Yoshiyuki,
Iwata Hiroji,
Mitani Masami,
Shinoda Noriyuki,
Sato Atsushi,
Mitsui Akira,
Sugiura Masahiko,
Kato Joji,
Fujii Yoshitaka
Publication year - 2002
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.10134
Subject(s) - matrix metalloproteinase , in vitro , gentamicin protection assay , cell culture , carcinoma , metastasis , zymography , cancer research , pathology , medicine , biology , cancer , biochemistry , genetics
Background and Objectives Although some investigators recently suggested that MMP‐9 may play a critical role in invasion and metastasis, along with MMP‐2, in esophageal carcinoma, there has been no direct evidence that MMPs play a critical role in the actual invasion of esophageal carcinoma cells. Here, we investigated the role of MMPs in the in vitro invasion of esophageal carcinoma cell lines (TE‐series). Methods Our methods included in vitro invasion assay, gelatin zymography, and enzyme‐linked immunosorbent assay (ELISA). Results Four cell lines (but not TE‐5) secreted MMP‐2 and MMP‐9 in the culture medium. Using a quantitative in vitro invasion assay, we found a significant ( P = 0.002) correlation between the extent of in vitro invasion and the amount of MMP‐9, but not of MMP‐2, secreted into the conditioned medium in the four cell lines. In these cell lines, R‐94138, a specific MMP‐9 inhibitor, inhibited in vitro invasion in a dose‐dependent manner. Although TE‐5 did not secrete MMP‐2 or MMP‐9, the cells showed a strong in vitro invasion. Conclusions Our data suggest that most of the esophageal carcinoma cell lines use MMP‐9 for in vitro invasion, but others may use proteinase(s) other than MMP‐9. J. Surg. Oncol. 2002;81:80–86. © 2002 Wiley‐Liss, Inc.