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Analysis of gene expression in Egr‐1 transfected human fibrosarcoma cells
Author(s) -
Liu YaGuang,
Gano Jacob,
Huang Ruochun,
Lin Ying,
Wang ShenMing,
Huang RuoPan
Publication year - 2002
Publication title -
journal of surgical oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.201
H-Index - 111
eISSN - 1096-9098
pISSN - 0022-4790
DOI - 10.1002/jso.10126
Subject(s) - transfection , gene , complementary dna , microbiology and biotechnology , gene expression , dna microarray , microarray , biology , transcription factor , reverse transcription polymerase chain reaction , genetics
Background and Objectives It has been shown previously that transcription factor Egr‐1 functions as a tumor suppressor gene. This study was conducted to investigate the possible mechanisms responsible for this function. Methods A cDNA microarray technique was applied to study differential gene expression between Egr‐1‐transfected cells and control‐transfected cells. Results A number of genes differentially expressed were identified. Among 5,184 genes examined by cDNA microarray technique, 53 were up‐regulated in Egr‐1 transfected cells, and 276 were down‐regulated in Egr‐1 transfected cells by at least two times or more; 13 genes were independently confirmed by reverse transcription polymerase chain reaction (RT‐PCR). Of these 13 genes, 11 were in agreement with cDNA microarrays in terms of gene expression levels. Conclusions Egr‐1 regulated almost every category of genes according to gene ontology system, and repressed more genes than it induced. The identification and further characterization of genes regulated by Egr‐1 should provide valuable information to understand Egr‐1 tumor suppressor action and the role of Egr‐1 as a transcription factor in general. J. Surg. Oncol. 2002;80:190–196. © 2002 Wiley–Liss, Inc.