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In vitro studies on the interactions of blood lipid level‐related biological molecules with gallic acid and tannic acid
Author(s) -
Zeng Xiangquan,
Sheng Zhongting,
Li Xiangxin,
Fan Xinguang,
Jiang Weibo
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9974
Subject(s) - tannic acid , chemistry , gallic acid , polyphenol , molecule , chromatography , electrospray ionization , hydrogen bond , solubility , molecular mass , biochemistry , mass spectrometry , organic chemistry , antioxidant , enzyme
Abstract BACKGROUND To provide the scientific evidence for a possible new mechanism of hypolipidemic effects of gallic acid (GA) and tannic acid (TA), the binding capacity of GA and TA with blood lipid level‐related biological molecules, including fat, cholesterol and cholates, were investigated in vitro . Additionally, we attempted to study the interactions of cholates with GA and TA by spectroscopic methods, high‐performance liquid chromatography electrospray‐ionization mass spectrometry (HPLC‐ESI‐MS) analysis and molecular modeling studies. RESULTS Our results demonstrated that both GA and TA were capable of binding with the blood lipid level‐related biological molecules in vitro . The fat‐binding capacity of TA was 122.1% that of GA when the addition of polyphenol was 90 mg. The inhibitory effects of GA and TA on the cholesterol solubility in mixed micelles and liquid egg yolk exhibited a dose‐dependent relationship (0.5–2.0 mg mL −1 ). In cholate‐binding tests, TA showed higher affinity for sodium cholate than GA at a concentration of 2.0 mg mL −1 , while no significant difference in the affinity for sodium deoxycholate was found between GA and TA. Moreover, the data of spectroscopic methods, HPLC‐ESI‐MS analysis and molecular modeling studies indicated that GA and TA might precipitate cholates through hydrophobic interactions and intermolecular hydrogen bonds rather than covalent bonds. CONCLUSION The findings of the present study suggested that the binding capacity of GA and TA with blood lipid level‐related biological molecules might play a crucial role in their hypolipidemic effects in animals. © 2019 Society of Chemical Industry