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Preparation and antagonistic effect of ACE inhibitory peptide from cashew
Author(s) -
Shu Yang,
Cao XianYing,
Chen Jian
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9967
Subject(s) - malondialdehyde , chemistry , glutathione , superoxide dismutase , peptide , pharmacology , renin–angiotensin system , inhibitory postsynaptic potential , angiotensin converting enzyme , enzyme , glutathione peroxidase , biochemistry , endocrinology , antioxidant , medicine , blood pressure
BACKGROUND Angiotensin‐converting enzyme (ACE) inhibitory peptides were found to alleviate acute hepatitis significantly. In this study, we purified and identified ACE inhibitory peptide from cashew to evaluate its protective role on alcohol‐induced acute hepatitis in mice. RESULTS The ACE inhibitory peptides were purified by using consecutive chromatographic techniques. One of these peptides (FETISFK) exhibited the highest ACE inhibition rate (91.04 ± 0.31%). In vivo , the results showed that ACE inhibitory peptide decreased levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) caused by alcohol exposure. Moreover, it could increase the activities of superoxide dismutase (SOD) and glutathione (GSH), and decrease the level of malondialdehyde (MDA). It was also found to down‐regulate markedly the expression of interleukin‐6 (IL‐6) and tumor necrosis factor‐ α (TNF‐ α ). It could also decrease the expression of ACE, angiotensin II (AngII) and angiotensin II type 1 receptor (AT 1 R). CONCLUSION These findings support the view that the ACE inhibitory peptide alleviated acute hepatitis by down‐regulating the ACE–AngII–AT 1 R axis, broadening the research approach to prevent acute hepatitis, and providing experimental data for the development and utilization of cashews. © 2019 Society of Chemical Industry