Premium
Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines
Author(s) -
He Dan,
Wu Siya,
Yan Liping,
Zuo Jihui,
Cheng Yang,
Wang Hanfei,
Liu Jian,
Zhang Xu,
Wu Mingjiang,
Choi Jongil,
Tong Haibin
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9954
Subject(s) - hela , chemistry , cell cycle , biochemistry , cancer cell , cell , microbiology and biotechnology , biology , cancer , genetics
BACKGROUND Pyropia yezoensis , rich in porphyran, is a medicine‐edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high‐yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated. RESULTS Pyropia yezoensis porphyran is a water‐soluble, triple‐helical sulfated hetero‐galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP‐20 and PYP‐50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP‐20 and PYP‐50. Three porphyrans had no toxicity in normal human liver cells (HL‐7702) and showed antitumor effects on Hep3B, HeLa and MDA‐MB‐231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5‐fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5‐fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin‐dependent kinase 1. CONCLUSION Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non‐toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry