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Relationship between the characteristics of rigor‐mortis‐related actomyosin and muscle fiber types in the ordinary muscle of various fishes
Author(s) -
Wang Yao,
Wang Qin,
Hirasaka Katsuya,
Hara Kenji,
Taniyama Shigeto,
Tachibana Katsuyasu
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9880
Subject(s) - rigor mortis , myosin , muscle fibre , dorsum , anatomy , fish <actinopterygii> , myosin atpase , chemistry , biophysics , biology , atpase , biochemistry , skeletal muscle , fishery , enzyme
Abstract BACKGROUND Rigor mortis occurs when muscle extension vanishes through the irresistible coupling of actin and myosin by the consumption of adenosine triphosphate as energy. To clarify the cause of the differences in the progression of rigor mortis, seven fish species were used as samples. The superprecipitation reaction and Mg 2+ ‐ATPase activity of actomyosin in dorsal ordinary muscle were measured, and the slope of the regression line between these two variables was calculated for each fish specimen. The fiber types of the dorsal ordinary muscle in each sample fish were discriminated by the stability of actomyosin ATPase at acid and alkaline preincubations. RESULT Positive correlations were found between Mg 2+ ‐ATPase activity and the superprecipitation reaction of actomyosin in all 27 fish specimens. The slopes of the regression lines were different not only between fish species but also in fish specimens within the same species. The area ratios of pink muscle fibers and the IIa and/or IIb subtypes of white muscle fibers in the dorsal ordinary muscle were also different between fish species, as well as in specimens within the same fish species. A positive correlation was found between the area ratios of pink muscle fibers in dorsal ordinary muscle and the slopes of the regression line. CONCLUSION It was suggested that the differences in characteristics of rigor‐mortis‐related actomyosin of fish might have been caused by the differences in the interposition ratio of muscle fiber types, especially of the pink muscle fiber type, in the dorsal ordinary muscle. © 2019 Society of Chemical Industry