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Anti‐inflammatory activity of β ‐caryophyllene combined with docosahexaenoic acid in a model of sepsis induced by Staphylococcus aureus in mice
Author(s) -
Brito Laís Ferraz,
Oliveira Hellen Braga Martins,
Neves Selis Nathan,
Souza Clarissa Leal Silva,
Júnior Manoel Neres Santos,
Souza Erika Pereira,
Silva Lucas Santana Coelho da,
Souza Nascimento Flávia,
Amorim Aline Teixeira,
Campos Guilherme Barreto,
Oliveira Márcio Vasconcelos,
Yatsuda Regiane,
Timenetsky Jorge,
Marques Lucas Miranda
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9861
Subject(s) - docosahexaenoic acid , sepsis , staphylococcus aureus , inflammation , polyunsaturated fatty acid , medicine , proinflammatory cytokine , oxidative stress , anti inflammatory , immunology , pharmacology , chemistry , microbiology and biotechnology , biology , fatty acid , biochemistry , bacteria , genetics
BACKGROUND Sepsis is a set of serious organic manifestations caused by an infection, whose progression culminates in exacerbated inflammation and oxidative stress, poor prognosis, and high hospital costs. Antioxidants used against sepsis have been evaluated, including essential oils such as β ‐caryophyllene (BCP), and polyunsaturated fatty acids, such as docosahexaenoic acid (DHA). The aim of this study was to evaluate the anti‐inflammatory activity of the association of these two compounds. RESULTS Treatment with BCP‐DHA, at a dose of 200 μL/animal, significantly inhibited the migration of neutrophils in a Cg‐induced peritonitis model. After Staphylococcus aureus infection, in the groups treated with BCP‐DHA there was a significant decrease in the total and differential count of leukocytes, increased expression of cytokines TNF‐ α and IFN‐ γ in treated groups, an increase of IL‐4 and IL‐5 in B/D and B/D + SA groups, and an augmentation of IL‐6 and IL‐12 groups in B/D + SA groups. Histological and bacterial analysis revealed lower neutrophil migration and lower bacterial load in the infected and treated groups. CONCLUSION In general, the BCP‐DHA association presented anti‐inflammatory activity against two different models of acute inflammation and infection, showing promising potential as a therapeutic adjuvant in sepsis. © 2019 Society of Chemical Industry