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Integration of proteome and transcriptome data reveals the mechanism involved in controlling of Fusarium graminearum by Saccharomyces cerevisiae
Author(s) -
Zhao Lina,
Cheng Yangyang,
Li Bo,
Gu Xiangyu,
Zhang Xiaoyun,
Boateng Nana Adwoa Serwah,
Zhang Hongyin
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9844
Subject(s) - saccharomyces cerevisiae , aldolase a , biology , biochemistry , proteome , glycolysis , phosphoglycerate mutase , transcriptome , yeast , citric acid cycle , proteomics , fusarium , glyceraldehyde 3 phosphate dehydrogenase , enzyme , dehydrogenase , gene , gene expression , genetics
BACKGROUND It has been reported that antagonistic microorganisms could effectively control the infection of Fusarium graminearum . However, there is limited information on the control of F. graminearum by Saccharomyces cerevisiae , while the possible control mechanisms involved through proteomic and transcriptomic techniques have also not been reported. RESULTS The results of this study showed that S. cerevisiae Y‐912 could significantly inhibit the growth of F. graminearum Fg1, and the spore germination rate and germ tube length of F. graminearum Fg1 were also significantly inhibited by S. cerevisiae Y‐912. Proteomic analysis revealed that differentially expressed proteins which were made of some basic proteins and enzymes related to basal metabolism, such as glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), phosphoglycerate mutase (PGAM), enolase (ENO), fructose diphosphate aldolase (FBA) and so on, were all down‐regulated. The transcriptomics of F. graminearum control by S. cerevisiae was also analyzed. CONCLUSION The control mechanism of S. cerevisiae Y‐912 on F. graminearum Fg1 was a very complex material and energy metabolic process in which the related proteins and genes involved in the glycolytic pathway, tricarboxylic acid (TCA) cycle and amino acid metabolism were all down‐regulated. © 2019 Society of Chemical Industry