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Selection of mutants with high linolenic acid contents and characterization of fatty acid desaturase 2 and 3 genes during seed development in soybean ( Glycine max )
Author(s) -
Hong Min Jeong,
Jang Young Eun,
Kim DongGun,
Kim Jung Min,
Lee MinKyu,
Kim JinBaek,
Eom Seok Hyun,
Ha BoKeun,
Lyu Jae Il,
Kwon SoonJae
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9798
Subject(s) - linolenic acid , polyunsaturated fatty acid , linoleic acid , mutant , biochemistry , biology , fatty acid , fatty acid desaturase , alpha linolenic acid , glycine , docosahexaenoic acid , gamma linolenic acid , gene , food science , amino acid
BACKGROUND Soybean seeds contain 18–24% lipids, which are made up of 85% polyunsaturated fatty acids. Two of these (linoleic and linolenic acids) comprise essential fatty acids that are not synthesized in humans and animals. Linolenic acid plays a vital role in the maintenance of brain function and is a source of docosahexaenoic acid for retinal and nerve tissue, with its physiological functions being a focus of attention. RESULTS We developed mutant soybean populations via gamma irradiation of Korean cultivars Danbaek and Daepung and evaluated the linolenic acid content of 78 and 154 M 9 mutant progenies. We selected the four mutant lines with the highest linolenic acid contents based on 2 years of investigation of fatty acids. The selected mutant lines had linolenic acid contents that were 33.9% to 67.7% higher than those of the original cultivars and exhibited increased fatty acid desaturase ( FAD ) gene expression levels during seed development. We also identified nucleotide polymorphisms of FAD genes in the four mutant lines. CONCLUSION The present study found that linolenic acid content is related to significantly increased expression levels of the FAD3C and FAD3D genes in the endoplasmic reticulum, which was uncovered by radiation mutation breeding of soybean. © 2019 Society of Chemical Industry