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A novel colorimetric immunosensor based on platinum colloid nanoparticles immobilized on PowerVision as signal probes and Fe 3 O 4 @ β ‐cyclodextrin as capture probes for ractopamine detection in pork
Author(s) -
Sun Yangying,
Fu Tian,
Chen Shuxian,
Wu Zhen,
Guo Yuxing,
Pan Daodong,
Gan Ning
Publication year - 2019
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9492
Subject(s) - ractopamine , detection limit , absorbance , chemistry , platinum nanoparticles , chromogenic , chromatography , colloid , nuclear chemistry , colorimetry , platinum , linear range , catalysis , organic chemistry
Background A novel colorimetric immunosensor was developed for the simple, sensitive and selective detection of ractopamine (RAC) based on using β ‐cyclodextrin‐modified Fe 3 O 4 particles (Fe 3 O 4 @ β ‐CD) as capture probes and complex platinum colloid nanoparticles (PtNPs‐PV) composed of platinum colloid nanoparticles (PtNPs) and polymerase chelate PowerVision (PV) as signal probes. Results PtNPs‐PV double catalyzed the chromogenic substrate 3,3′‐diaminobenzidine (DAB), which induced changes in the color of DAB and chromogenic absorbance. Incubation temperature, pH and incubation time were systematically optimized and, under optimum conditions, the measured absorbance values showed a linear relationship with the RAC concentrations in the range 0.03–8.1 ng mL −1 . The detection limit was 0.01 ng mL −1 . The sensor exhibited high sensitivity and specificity, as demonstrated by testing structurally similar organic compounds such as salbutamol, clenbuterol and dopamine. The practicality of the developed colorimetric immunosensor was supported by the successful detection of RAC in pork samples with recovery ranging from 94.00% to 106.00%. Conclusion We designed a novel sandwich‐type noncompetitive colorimetric immunoassay for the detection of trace levels of RAC in pork. The proposed method can also be used for the detection of toxins in food products via PtNPs‐PV amplification. © 2018 Society of Chemical Industry