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Functional and structural properties of red color‐related pigment‐binding protein from the shell of Litopenaeus vannamei
Author(s) -
Pan Chuang,
Ishizaki Shoichiro,
Nagashima Yuji,
Watabe Shugo
Publication year - 2018
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9361
Subject(s) - hemocyanin , hepatopancreas , litopenaeus , hemolymph , pigment , shrimp , biochemistry , biology , astaxanthin , shell (structure) , biophysics , chemistry , carotenoid , ecology , genetics , materials science , organic chemistry , composite material , antigen
BACKGROUND A novel red color‐related pigment‐binding protein named LvPBP75 isolated from the shell of Litopenaeus vannamei has recently been identified as hemocyanin. However, information on the functional and structural properties of LvPBP75 is insufficient. This study aimed to elucidate the thermal properties and pigment‐binding ability of LvPBP75. RESULTS LvPBP75 showed significant red color change after heat treatment with high concentrations of NaCl (>0.1 mol L −1 ), acidic (<5) or alkaline (>9) pH values and alcohols. LvPBP75 mRNA expression analysis revealed that expression level was highest in hepatopancreas and weakest in muscle. Reconstruction and structural analysis revealed that astaxanthin could bind to hemocyanin derived from the shell of L. vannamei but not to hemocyanins derived from the hepatopancreas or hemolymph of other invertebrates. Three‐dimensional models of hemocyanin monomer displayed significant structural differences between native LvPBP75 and hemocyanin derived from shrimp hepatopancreas. CONCLUSION The results suggest a novel function of hemocyanin as binding with pigment and its involvement in L. vannamei shell color change. The pigment‐binding ability of hemocyanins has species and tissue specificity, and their unique structural features play an important role in binding ability. © 2018 Society of Chemical Industry