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Ornithine decarboxylase is involved in methyl jasmonate‐regulated postharvest quality retention in button mushrooms ( Agaricus bisporus )
Author(s) -
Meng DeMei,
Wang HuaDong,
Zhang YaXuan,
Xi ZhiAi,
Yang Rui,
Sheng JiPing,
Zhang XinHua,
Ding Yang,
Wang JunPing,
Fan ZhenChuan
Publication year - 2018
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.9247
Subject(s) - methyl jasmonate , agaricus bisporus , ornithine decarboxylase , postharvest , chemistry , catalase , putrescine , polyphenol oxidase , superoxide dismutase , biochemistry , food science , peroxidase , horticulture , mushroom , biology , antioxidant , enzyme , gene
BACKGROUND In the present study, we investigated the role of ornithine decarboxylase (ODC) in the methyl jasmonate (MeJA)‐regulated postharvest quality maintenance of Agaricus bisporus (J. E. Kange) Imbach button mushrooms by pretreating mushrooms with a specific irreversible inhibitor called α ‐difluoromethylornithine (DFMO) before exposure to MeJA vapor. RESULTS Mushrooms were treated with 0 or 100 µmol L −1 MeJA or a combination of 120 µmol L −1 DFMO and 100 µmol L −1 MeJA, respectively, before storage at 4 °C for 21 days. Treatment with MeJA alone induced the increase in ODC activity whereas this effect was greatly suppressed by pretreatment with DFMO. α ‐Difluoromethylornithine strongly attenuated the effect of MeJA on decreasing cap opening, slowing the decline rate of soluble protein and total sugar, and accumulating total phenolics and flavonoids. α ‐Difluoromethylornithine pretreatment also counteracted the ability of MeJA to inhibit polyphenol oxidase and lipoxygenase activities, and malondialdehyde production, and to stimulate superoxide dismutase and catalase activities. It also largely downregulated MeJA‐induced accumulation of free putrescine (Put). CONCLUSION These results reveal that ODC is involved in MeJA‐regulated postharvest quality retention of button mushrooms, and this involvement is likely to be associated with Put levels. © 2018 Society of Chemical Industry