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The presence of bioactive peptides in hydrolysates prepared from processing waste of sardine ( Sardina pilchardus )
Author(s) -
RavallecPlé Rozenn,
Charlot Caroline,
Pires Carla,
Braga Vera,
Batista Irineu,
Van Wormhoudt Alain,
Le Gal Yves,
FouchereauPéron Martine
Publication year - 2001
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.921
Subject(s) - sardine , hydrolysate , chemistry , peptide , chromatography , enzyme , biochemistry , food science , hydrolysis , biology , fish <actinopterygii> , fishery
Abstract A proteolytic enzyme, Alcalase®, was used to produce partly digested proteins from cooked wastes of sardine ( Sardina pilchardus ). The presence of biologically active molecules was investigated in these hydrolysates prepared under various conditions of time and enzyme/substrate ratio. By means of radioimmunoassays and mitogenic and radioreceptor assays, the presence of molecules related to secretagogue peptides, growth factors and calcitonin gene‐related peptide (CGRP) respectively was detected in the hydrolysates. Exclusion chromatography permits the conclusion that the biological activity of the different fractions is related to their size. © 2001 Society of Chemical Industry