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Preparative isolation and purification of capsaicin and dihydrocapsaicin from Capsici Fructus using supercritical fluid extraction combined with high speed countercurrent chromatography
Author(s) -
Yan Rongwei,
Zhao Leilei,
Tao Junfei,
Zou Yong,
Xu Xinjun
Publication year - 2018
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8736
Subject(s) - chromatography , countercurrent exchange , extraction (chemistry) , capsaicin , chemistry , supercritical fluid , supercritical fluid extraction , countercurrent chromatography , isolation (microbiology) , high performance liquid chromatography , organic chemistry , biology , biochemistry , receptor , anatomy , microbiology and biotechnology
BACKGROUND Supercritical fluid extraction with CO 2 (SFE‐CO 2 ) was utilized for extraction of capsaicin (CA) and dihydrocapsaicin (DHCA) from Capsici Fructus, and then a two‐step enrichment method for separating capsaicinoids from SFE‐CO 2 extracts was developed. The process involved extraction with aqueous methanol and crystallization by alkali extraction and acid precipitation. Finally, a consecutive high‐speed countercurrent chromatography (HSCCC) separation method was successfully applied in the purification of CA and DHCA from capsaicinoid crystal. RESULTS The extraction pressure, extraction temperature and volume of co‐solvent were optimized at 33 MPa, 41 °C and 75 mL, respectively, using response surface methodology; the extraction rates of CA and DHCA were about 93.18% and 93.49%, respectively. 407.43 mg capsaicinoid crystal was isolated from the SFE‐CO 2 extracts obtained from 100 g capsicum powder by the two‐step enrichment method. About 506 mg and 184 mg CA and DHCA with purities up to 98.31% and 96.68%, respectively, were obtained from 1 g capsaicinoid crystal in one HSCCC of three consecutive sample loadings without exchanging any solvent system. CONCLUSIONS This method comprising SFE‐CO 2 , a two‐step enrichment and HSCCC was efficient, powerful and practical for the large‐scale preparation of CA and DHCA from Capsici Fructus with high purity and high yield. © 2017 Society of Chemical Industry