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Physicochemical characterization of actomyosin–paramyosin from giant squid mantle ( Dosidicus gigas )
Author(s) -
TolanoVillaverde Ivan J,
OcañoHiguera Victor,
EzquerraBrauer Josafat,
SantosSauceda Irela,
SantacruzOrtega Hisila,
CárdenasLópez José L,
RodríguezOlibarria Guillermo,
MárquezRíos Enrique
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8653
Subject(s) - myosin , myofibril , squid , tropomyosin , chemistry , mantle (geology) , amino acid , actin , biochemistry , biology , fishery , paleontology
BACKGROUND The giant squid ( Dosidicus gigas ) has been proposed as raw material to obtain myofibrillar protein concentrates. However, it has been observed that colloidal systems formed from squid proteins have limited stability. Therefore, the isolation and characterization of the actomyosin–paramyosin isolated (API) complex were performed, because they are the main proteins to which functionality has been attributed. RESULTS Densitogram analysis revealed 45% of actin, 38% of myosin and 17% of paramyosin. The amino acid profile indicates a higher proportion of acidic amino acids, which gives a higher negative charge; this was supported by the zeta potential. Total sulfhydryl (TSH) content was lower compared with proteins of other aquatic species. CONCLUSION The higher percentage of actin in relation to myosin, the presence of paramyosin, as well as the low content of sulfhydryl groups, could comprise the main causes of the low technological functional property of proteins from D. gigas mantle. © 2017 Society of Chemical Industry