Premium
Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia
Author(s) -
Thong Kwai Lin,
Tan Lai Kuan,
Ooi Peck Toung
Publication year - 2018
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8442
Subject(s) - yersinia enterocolitica , virulence , tetracycline , microbiology and biotechnology , biology , ampicillin , yersinia , antibiotic resistance , nalidixic acid , multiple drug resistance , antimicrobial , antibiotics , bacteria , gene , genetics
BACKGROUND The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS Thirty‐two non‐repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B /O:8, n = 3; 1A /O:5, n = 2) were analysed. Approximately 90% of strains were multidrug‐resistant with a multiple antibiotic resistance index < 0.2 and the majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin. Yersinia enterocolitica could be distinguished distinctly into three clusters by pulsed‐field gel electrophoresis, with each belonging to a particular bioserotype. Strains of 3 variant/O:3 were more heterogeneous than others. Eleven of the 15 virulence genes tested ( hreP , virF , rfbC , myfA , sat , inv , ail , ymoA , ystA , tccC , yadA ) and pYV virulence plasmid were present in all the bioserotpe 3 variant/03 strains. CONCLUSION The occurrence of virulent strains of Y. enterocolitica in pigs and porcine products reiterated that pigs are important reservoirs for Y. enterocolitica. The increasing trend of multidrug resistant strains is a public health concern. This is the first report on the occurrence of potential pathogenic and resistant strains of Y. enterocolitica in pigs in Malaysia. © 2017 Society of Chemical Industry