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Determination of oral bioavailability of curcuminoid dispersions and nanoemulsions prepared from Curcuma longa Linnaeus
Author(s) -
Lu Pei Shan,
Inbaraj Baskaran Stephen,
Chen Bing Huei
Publication year - 2018
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8437
Subject(s) - curcuminoid , curcumin , bioavailability , curcuma , chemistry , chromatography , pharmacokinetics , high performance liquid chromatography , pharmacology , traditional medicine , biochemistry , medicine
BACKGROUND Curcuminoid from Curcuma longa Linnaeus has been demonstrated to be effective in anti‐cancer and anti‐inflammation. The objectives of the present study were to prepare curcuminoid dispersion and nanoemulsion from C. longa and determine their oral bioavailabilities in rats. RESULTS After curcuminoid extraction using 99.5% ethanol, bisdemethoxycurcumin ( BDMC ), demethoxycurcumin ( DMC ) and curcumin were separated within 10 min by high‐performance liquid chromatography using an Eclipse XDB‐C18 column (Agilent, Palo Alto, CA , USA ) and a gradient mobile phase of 0.1% aqueous formic acid and acetonitrile, with a flow rate of 1 mL min −1 , column temperature of 35 °C and detection wavelength of 425 nm. Curcuminoid nanoemulsion at a particle size of 12.1 nm and encapsulation efficiency 98.8% was prepared using lecithin, Tween 80 and water. A pharmacokinetic study in rats revealed that the parameters including T max , C max , t 1/2 and the area under the curve were higher for curcuminoid nanoemulsions than for curcuminoid dispersion at the same dose employed for gavage administration, whereas, for intravenous injection, an opposite trend was shown. The oral bioavailabilities of BDMC , DMC , curcumin and total curcuminoids in nanoemulsion and dispersion were 34.39 and 4.65%, 39.93 and 5.49%, 47.82 and 9.38%, and 46 and 8.7%, respectively. CONCLUSION The results of the present study demonstrate a higher oral bioavailability after incorporation of curcuminoid into nanoemulsion, facilitating its application as a botanic drug. © 2017 Society of Chemical Industry