Premium
Highly sensitive method for the quantification of trans ‐linolenic acid isomers in trilinolenin of edible oils using an ionic liquid capillary column
Author(s) -
Guo Qin,
Jiang Fan,
Jin Jing,
Li Qingpeng,
Wang Feng,
Wang Qiang,
Ha Yiming
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8337
Subject(s) - chemistry , chromatography , flame ionization detector , isomerization , detection limit , reproducibility , ionic liquid , gas chromatography , capillary action , linolenic acid , analytical chemistry (journal) , organic chemistry , linoleic acid , materials science , fatty acid , catalysis , composite material
BACKGROUND The polarities of linolenic acid isomers are very similar, and only a few studies to date have attempted to separate α‐linolenic acid ( ALA ) isomers completely. The aim of this study was to fill this gap by developing and validating an accurate method for the analysis of ALA isomers in trilinolenin at 200, 220 and 240 °C using a gas chromatograph–flame ionization detector equipped with an SLB‐IL111 capillary column. RESULTS Results showed that eight ALA isomer standards were separated effectively using these optimized gas chromatographic conditions. The coefficient of determination was r 2 > 0.9994 in the linear range of each ALA isomer. The obtained limits of detection and limits of quantification of the ALA isomers were 0.02–0.08 ppm and 0.05–0.22 ppm, respectively. A high degree of reproducibility and percent recoveries between 96.2% and 106.5%, with coefficients of variation ranging from 0.82% to 0.97%, were achieved. CONCLUSION The developed method has been successfully applied to the analysis of ALA isomers in heated pure trilinolenin as well as to trilinolenin in various edible oils, and the TALA isomerization pathways in heated trilinolenin were verified. © 2017 Society of Chemical Industry