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Hen egg yolk phosvitin stimulates osteoblast differentiation in the absence of ascorbic acid
Author(s) -
Liu Qingli,
Li Chunyan,
Geng Fang,
Huang Xi,
Ma Meihu
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8320
Subject(s) - phosvitin , ascorbic acid , osteoblast , alkaline phosphatase , chemistry , osteocalcin , biochemistry , staining , yolk , alizarin , microbiology and biotechnology , biology , enzyme , genetics , protein kinase a , food science , organic chemistry , in vitro
BACKGROUND Egg yolk phosvitin, one of the most highly phosphorylated extracellular matrix proteins known in nature, has a strong calcium binding and reducing capacity. Here, we investigated the effects of phosvitin on osteoblast differentiation and osteogenic gene expression in cultured mouse osteoblastic MC3T3‐E1 cells by using alkaline phosphatase activity analysis, alizarin red S staining and real‐time PCR assay. RESULTS Alkaline phosphatase activity and alizarin red S staining analyses demonstrated no significant difference between differentiating MC3T3‐E1 cells cultured in the presence of phosvitin and those cultured in the presence of ascorbic acid after 21 days of differentiation. Our real‐time PCR assay also indicated the two groups were similar in the expression of the osteogenic gene markers, collagen type I, osteocalcin, runt‐related transcription factor 2, and bone morphogenetic protein‐2. CONCLUSION Our findings indicate that phosvitin plays a similar role to that of ascorbic acid in osteoblast differentiation and mineralisation. © 2017 Society of Chemical Industry