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Enzymatic hydrolysis of Grass Carp fish skin hydrolysates able to promote the proliferation of Streptococcus thermophilus
Author(s) -
Wang XiaoNan,
Qin Mei,
Feng YuYing,
Chen JianKang,
Song YiShan
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8299
Subject(s) - hydrolysate , streptococcus thermophilus , hydrolysis , grass carp , food science , chemistry , enzymatic hydrolysis , carp , neutral protease , enzyme , common carp , biochemistry , fish <actinopterygii> , protease , biology , fishery , fermentation , cyprinus , lactobacillus
BACKGROUND The promotion effect on proliferation of Streptococcus thermophilus by enzymatic hydrolysates of aquatic products was firstly studied. The effect of influencing factors of the hydrolysis on the growth of S. thermophilus was investigated. RESULT Grass Carp fish skin was hydrolysed to peptides by enzymatic hydrolysis using protease ProteAX , and for the S. thermophilus growth, the optimal enzymatic hydrolysis conditions were temperature of 60 °C, initial pH of 9.0, enzyme concentration of 10 g kg −1 , hydrolysis time of 80 min, and ratio of material to liquid of 1:2. The Grass Carp fish skin hydrolysate ( GCFSH ) prepared under the optimum conditions was fractionated to five fragments ( GCFSH 1, GCFSH 2, GCFSH 3, GCFSH 4, GCFSH 5) according to molecular weight sizes, in which the fragments GCFSH 4 and GCFSH 5, with molecular weights of less than 1000 Da, significantly promoted the growth of S. thermophilus . CONCLUSION The hydrolysis process of Grass Carp fish skin can be simplified, and the peptides with molecular weights below 1000 Da in the hydrolysates are the best nitrogen source for proliferation of S. thermophilus . This work can provide a fundamental theoretical basis for the production of multi‐component functional foods, especially in milk drinks or yogurt. © 2017 Society of Chemical Industry