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Purification of Lactobacillus acidophilus surface‐layer protein and its immunomodulatory effects on RAW264 .7 cells
Author(s) -
Zhang Dandan,
Wu Mengting,
Guo Yuxing,
Xun Mingyue,
Wang Wenwen,
Wu Zhen,
Pan Daodong
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8294
Subject(s) - lactobacillus acidophilus , chemistry , circular dichroism , gel electrophoresis , polyacrylamide gel electrophoresis , lactobacillus , tumor necrosis factor alpha , cell , secretion , food science , sodium dodecyl sulfate , biochemistry , bacteria , microbiology and biotechnology , biology , probiotic , immunology , fermentation , genetics , enzyme
BACKGROUND Surface‐layer proteins ( SLP ) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity. RESULTS The present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP . SLP were derived from Lactobacillus acidophilus CICC6074 with a molecular weight of 46 kDa , and consisted of 16.9% α‐helix, 42.3% β‐sheet, 20.8% β‐turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50 µg mL −1 significantly decreased the amount of tumor necrosis factor‐α secreted by RAW264 .7 cells. CONCLUSION SLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L. acidophilus in food, medicine and other products. © 2017 Society of Chemical Industry