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Determination of two potential toxicity metabolites derived from the disruption of the pksCT gene in Monascus aurantiacus Li As3.4384
Author(s) -
Huang Zhibing,
Su Baowei,
Xu Yang,
Li Laisheng,
Li Yanping
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8291
Subject(s) - monascus , extraction (chemistry) , citrinin , chromatography , chemistry , relative standard deviation , fermentation , methanol , high performance liquid chromatography , mycotoxin , food science , detection limit , organic chemistry
BACKGROUND We previously demonstrated that disruption of the pksCT gene of Monascus led to a greater than 98% decrease in its citrinin production capacity in Monascus (PHDS26). Two potentially toxic compounds, monascopyridine A (MPA) and monascopyridine B (MPB), were found in the fermentation products of the pksCT gene‐disrupted Monascus . Moreover, a rapid and reliable high‐performance liquid chromatography method was developed for the simultaneous determination of MPA and MPB. We studied the effects of various extraction parameters and designed an orthogonal experiment to investigate the importance of each factor. RESULTS The optimal extraction conditions were: methanol concentration, 90%; extraction temperature, 40 °C; extraction time, 10 min; two extraction cycles; and a solid‐liquid ratio of 1:25. Under the optimal chromatographic conditions, good linearity was reached over the concentration ranges 0.5–200 µg mL −1 and 0.5–300 µg mL −1 for MPA and MPB, respectively, and the corresponding determination coefficients were 0.9999 and 0.9997. The percentage relative standard deviation values of within‐day and between‐day precision for MPA were 2.0% and 2.1%, respectively; the corresponding values for MPB were 4.8% and 4.6%. The average recovery for MPA and MPB was 99.9% and 94%, respectively. CONCLUSION Maximum MPA and MPB yields (2073.7 and 1961.7 µg g −1 , respectively) were observed after 16 days of cultivation. © 2017 Society of Chemical Industry