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Application and bioactive properties of CaTI , a trypsin inhibitor from Capsicum annuum seeds: membrane permeabilization, oxidative stress and intracellular target in phytopathogenic fungi cells
Author(s) -
Silva Marciele S,
Ribeiro Suzanna FF,
Taveira Gabriel B,
Rodrigues Rosana,
Fernandes Katia VS,
Carvalho André O,
Vasconcelos Ilka Maria,
Mello Erica Oliveira,
Gomes Valdirene M
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8243
Subject(s) - biology , protease , reactive oxygen species , microbiology and biotechnology , fusarium oxysporum , antimicrobial , biochemistry , protease inhibitor (pharmacology) , trypsin inhibitor , trypsin , botany , enzyme , human immunodeficiency virus (hiv) , antiretroviral therapy , viral load , immunology
Abstract BACKGROUND During the last few years, a growing number of antimicrobial peptides have been isolated from plants and particularly from seeds. Recent results from our laboratory have shown the purification of a new trypsin inhibitor, named CaTI , from chilli pepper ( Capsicum annuum L.) seeds. This study aims to evaluate the antifungal activity and mechanism of action of CaTI on phytopathogenic fungi and detect the presence of protease inhibitors in other species of this genus. RESULTS Our results show that CaTI can inhibit the growth of the phytopathogenic fungi Colletotrichum gloeosporioides and C. lindemuthianum . CaTI can also permeabilize the membrane of all tested fungi. When testing the inhibitor on its ability to induce reactive oxygen species, an induction of reactive oxygen species ( ROS ) and nitric oxide ( NO ) particularly in Fusarium species was observed. Using CaTI coupled to fluorescein isothiocyanate ( FITC ), it was possible to determine the presence of the inhibitor inside the hyphae of the Fusarium oxysporum fungus. The search for protease inhibitors in other Capsicum species revealed their presence in all tested species. CONCLUSION This paper shows the antifungal activity of protease inhibitors such as CaTI against phytopathogenic fungi. Antimicrobial peptides, among which the trypsin protease inhibitor family stands out, are present in different species of the genus Capsicum and are part of the chemical arsenal that plants use to defend themselves against pathogens. © 2017 Society of Chemical Industry