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Extraction, partial purification and characterization of amylase from parthenocarpic date ( Phoenix dactylifera ): effect on cake quality
Author(s) -
El Abed Hanen,
Khemakhem Bassem,
Fendri Imen,
Chakroun Mouna,
Triki Mehdi,
Drira Noureddine,
Mejdoub Hafedh
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8198
Subject(s) - phoenix dactylifera , parthenocarpy , amylase , food science , horticulture , enzyme , chemistry , botany , biology , palm , biochemistry , physics , quantum mechanics
BACKGROUND Phoenix dactylifera L. plays an important role in social, economic and ecological Tunisian sectors. Some date palms produce parthenocarpic fruit named Sish. The objective of the present study was to extract biomolecules from parthenocarpic fruit by producing value‐added products from the fruits. RESULTS The extraction of amylolytic activity from parthenocarpic fruit ( AmyPF ) was optimized using Box–Behnken design ( BBD ). Partial purification of about 250‐fold with an activity yield of 47% was achieved. The amylase exhibited a specific activity of 80 U mg −1 protein. The optimum pH and temperature for enzyme activity were 5 and 55 °C respectively. The enzyme was highly active over a wide range of pH (5–10), and significant stabilization was observed at 60 °C. The purified enzyme belongs to the exo type of amylases. Given the economic and industrial relevance of amylases used in the food industry, three different concentrations of AmyPF (0.007, 0.014 and 0.018 U g −1 ) were incorporated into a cake formulation, resulting in a decrease in density, moisture retention and water activity and an increase in hardness. CONCLUSION The beneficial effect of AmyPF on the technological characteristics of cakes was confirmed by sensory evaluation. © 2017 Society of Chemical Industry