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Production of chlorothalonil hydrolytic dehalogenase from agro‐industrial wastewater and its application in raw food cleaning
Author(s) -
He Qin,
Xu Xihui,
Zhang Fan,
Tai Yukai,
Luo Yanfei,
He Jian,
Hong Qing,
Jiang Jiandong,
Yan Xin
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8079
Subject(s) - wastewater , fermentation , pulp and paper industry , corn steep liquor , chemistry , chlorothalonil , raw material , food science , industrial wastewater treatment , industrial fermentation , waste management , environmental science , agronomy , environmental engineering , biology , organic chemistry , pesticide , engineering
BACKGROUND To reduce the fermentation cost for industrialization of chlorothalonil hydrolytic dehalogenase (Chd), agro‐industrial wastewaters including molasses, corn steep liquor ( CSL ) and fermentation wastewater were used to substitute for expensive carbon and nitrogen sources and fresh water for lab preparation. RESULTS The results showed that molasses and CSL could replace 5% carbon source and 100% organic nitrogen source respectively to maintain the same fermentation level. Re‐fermentation from raffinate of ultra‐filtered fermentation wastewater could achieve 61.03% of initial Chd activity and reach 96.39% activity when cultured in a mixture of raffinate and 50% of original medium constituent. Typical raw foods were chosen to evaluate the chlorothalonil removal ability of Chd. After Chd treatment for 2 h at room temperature, 97.40 and 75.55% of 30 mg kg −1 chlorothalonil on cherry tomato and strawberry respectively and 60.29% of 50 mg kg −1 chlorothalonil on Chinese cabbage were removed. Furthermore, the residual activity of the enzyme remained at 78–82% after treatment, suggesting its potential for reuse. CONCLUSION This study proved the cost‐feasibility of large‐scale production of Chd from agro‐industrial wastewater and demonstrated the potential of Chd in raw food cleaning. © 2016 Society of Chemical Industry