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Characterization of peptides from common bean protein isolates and their potential to inhibit markers of type‐2 diabetes, hypertension and oxidative stress
Author(s) -
Mojica Luis,
LunaVital Diego A,
González de Mejía Elvira
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8053
Subject(s) - oxidative stress , antioxidant , dipeptidyl peptidase , pepsin , digestion (alchemy) , enzyme , diabetes mellitus , chemistry , population , trolox , type 2 diabetes , food science , biochemistry , biology , antioxidant capacity , medicine , endocrinology , chromatography , environmental health
BACKGROUND Diabetes and hypertension are diseases affecting a high proportion of the world population; the use of food‐based products such as common bean peptides may contribute to reduce the risk of complications associated to chronic diseases. The aim was to produce and characterize peptides from common bean protein isolates and evaluate their potential to inhibit markers of type‐2 diabetes, hypertension and oxidative stress. RESULTS Mexican black and Brazilian Carioca bean isolated proteins were characterized after pepsin/pancreatin digestion. Also, four synthesized pure peptides, originally found in these beans, were evaluated. Bean protein digests and pure peptides exerted dipeptidyl peptidase‐ IV ( DPP‐IV ) inhibition ( IC 50 = 0.03–0.87 mg dry weight ( DW ) mL −1 ). Lineweaver–Burk plots and computational modeling showed competitive inhibition of DPP‐IV . Angiotensin‐converting enzyme ( ACE ) inhibition ranged from IC 50 = 0.09 to 0.99 mg DW mL −1 , and α ‐glucosidase inhibition ranged from 36.3 to 50.1% mg −1 DW . Carioca Perola bean digested proteins presented the highest antioxidant capacity (269.3 mmol L −1 Trolox equivalent g −1 DW ) as the peptide KTYGL ( P > 0.05) with the most potent DPP‐IV and ACE inhibition. CONCLUSION Peptides from common bean have antidiabetic and antihypertensive potential regardless of their antioxidant capacity. © 2016 Society of Chemical Industry