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Colloidal gold‐ McAb probe‐based rapid immunoassay strip for simultaneous detection of fumonisins in maize
Author(s) -
Yao Jingjing,
Sun Yaning,
Li Qingmei,
Wang Fangyu,
Teng Man,
Yang Yanyan,
Deng Ruiguang,
Hu Xiaofei
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.8032
Subject(s) - fumonisin b1 , chromatography , mycotoxin , chemistry , colloidal gold , detection limit , immunoassay , assay sensitivity , fumonisin , antibody , nanoparticle , biology , nanotechnology , medicine , materials science , alternative medicine , food science , pathology , immunology
Abstract BACKGROUND Fumonisins are a kind of toxic and carcinogenic mycotoxin. A rapid immunochromatographic test strip has been developed for simultaneous detection of fumonisin B 1 , B 2 and B 3 ( FB 1 , FB 2 and FB 3 ) in maize based on colloidal gold‐labelled monoclonal antibody ( McAb ) against FB 1 probe. RESULTS The anti‐ FB 1 McAb ( 2E11‐H3 ) was produced through immunisation and cell fusion, and identified as high affinity, specificity and sensitivity. The cross‐reaction ratios with fumonisin B 2 and B 3 were accordingly 385% and 72.4%, while none with other analogues. The colloid gold‐labelled anti‐ FB 1 McAb probe was successfully prepared and used for establishing the immunochromatographic strip. The test strip showed high sensitivity and specificity, the IC 50 for FB 1 was 58.08 ng mL −1 , LOD was 11.24 ng mL −1 , calculated from standard curve. Moreover, the test strip exhibited high cross‐reactivity with FB 2 and FB 3 , and could be applied to the simultaneous detection of FBs ( FB 1 : FB 2 : FB 3 = 12:4:1) in maize sample with high accuracy and precision. The average recoveries of FBs in maize ranged from 90.42% to 95.29%, and CVs were 1.25–3.77%. The results of the test strip for FBs samples showed good correlation with high‐performance liquid chromatography analysis. CONCLUSION The immunochromatographic test strip could be employed in the rapid simultaneous detection of FB 1 , FB 2 and FB 3 in maize samples on‐site. © 2016 Society of Chemical Industry