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Characteristics of lactic acid bacteria isolates and their effect on the fermentation quality of Napier grass silage at three high temperatures
Author(s) -
Gulfam Ali,
Guo Gang,
Tajebe Seare,
Chen Lei,
Liu Qinhua,
Yuan Xianjun,
Bai Yunfeng,
Saho Tao
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7998
Subject(s) - silage , fermentation , lactic acid , microbial inoculant , pediococcus acidilactici , pennisetum purpureum , food science , butyric acid , biology , lactic acid fermentation , chemistry , agronomy , bacteria , lactobacillus plantarum , dry matter , genetics
BACKGROUND The poor fermentation quality of silage is an important issue for silage production during the high temperatures of summer. Pediococcus acidilactici GG13 ( GG13 ) and Lactobacillus rhamnosus GG26 ( GG26 ) isolated from Italian ryegrass ( Lolium multiflorum Lam.) silage were characterised by morphological and physiological tests and 16S rRNA sequencing analysis, and their effects, along with those of a commercial lactic acid bacteria ( LAB ) inoculant ( CB ), on the fermentation quality of facultative halophyte Napier grass ( Pennisetum purpureum Schumach) ensiled at 30 °C, 40 °C and 50 °C were studied, respectively. RESULT The strains GG13 and GG26 grew well at 50 °C and pH 3.5, and were tolerant to 6.5% NaCl . After ensiling for 50 days, the strains GG13 and GG26 and the CB decreased ( P < 0.001) the pH and acetic acid and ammonia‐N contents and increased ( P < 0.001) the lactic acid contents at 30 °C, and decreased ( P < 0.001) the ammonia‐N contents at 40 °C in Napier grass. CB did not affect the fermentation quality at 50 °C, whereas both isolated strains improved the fermentation quality of Napier grass silage as indicated by the lower ( P < 0.001) pH , butyric acid and ammonia‐N contents and higher ( P < 0.001) lactic acid contents. The strain GG13 is better than GG26 with regard to improvement in fermentation quality of Napier grass silage. CONCLUSIONS The results of this study suggested that strain GG13 is a good LAB inoculant for producing well‐fermented silages during the high temperatures of summer times. © 2016 Society of Chemical Industry