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High‐sensitivity chemiluminescent immunoassay investigation and application for the detection of T‐2 toxin and major metabolite HT ‐2 toxin
Author(s) -
Li Yanshen,
Zhang Jing,
Mao Xin,
Wu Yongtao,
Liu Gongzhen,
Song Liting,
Li Yonggang,
Yang Jianrong,
You Yanli,
Cao Xuelin
Publication year - 2017
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7801
Subject(s) - toxin , metabolite , chemiluminescence , mycotoxin , immunoassay , chemistry , chemiluminescent immunoassay , chromatography , trichothecene , substrate (aquarium) , detection limit , biology , biochemistry , food science , antibody , immunology , ecology
BACKGROUND T‐2 toxin is a widely distributed mycotoxin in cereals. HT ‐2 toxin is the major metabolite, which is also a contaminant in cereals. T‐2 toxin and HT ‐2 toxin have been identified as having carcinogenic, hepatotoxic, teratogenic and immunotoxic properties. To reduce the risk of contamination, a rapid, highly sensitive and inexpensive assay for the detection is required. RESULTS In this study a high‐sensitivity chemiluminescent enzyme‐linked immunoassay ( CL‐ELISA ) of T‐2 toxin and HT ‐2 toxin was developed. With the help of the chemiluminescent substrate, this protocol showed a highly sensitive character with an IC 50 as low as 33.28 ng mL −1 and 27.27 ng mL −1 for T‐2 and HT ‐2, respectively. In addition, this method had no cross‐reaction with other structurally related mycotoxins. CONCLUSION These results indicated that the developed CL‐ELISA could be applied for the detection of T‐2 toxin and HT ‐2 toxin in actual samples without complicated steps. © 2016 Society of Chemical Industry