Premium
Sequence characterization and in silico structure prediction of fatty acid desaturases in linseed varieties with differential fatty acid composition
Author(s) -
Rajwade Ashwini V,
Joshi Rakesh S,
Kadoo Narendra Y,
Gupta Vidya S
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7775
Subject(s) - gene isoform , in silico , biology , fatty acid , gene , fatty acid desaturase , biochemistry , nucleotide , peptide sequence , linolenic acid , linseed oil , oleic acid , linoleic acid , food science , polyunsaturated fatty acid
BACKGROUND Linseed is the richest agricultural source of α ‐linolenic acid ( ALA ), an ω ‐3 fatty acid ( FA ) that offers several nutritional benefits. In the present study, sequence characterization of six desaturase genes ( SAD1 , SAD2 , FAD2 , FAD2 ‐2 , FAD3A and FAD3B ) and 3D structure prediction of their proteins from ten Indian linseed varieties differing in ALA content were performed to determine whether the nucleotide and amino acid ( AA ) sequence variants have any functional implications in differential accumulation of ALA or other FAs in linseed. RESULTS The SAD and FAD2 genes exhibited few sequence variations among the ten varieties, forming only one or two protein isoforms. In contrast, the FAD3A and FAD3B genes showed more sequence variations and three or four protein isoforms. Interestingly, the two high‐ ALA varieties NL260 and Padmini had the same FAD3B nucleotide and protein isoforms, which differed from all other varieties. Surprisingly, no AA changes altered the 3D structures of the desaturase proteins. CONCLUSION Several nucleotide and AA sequence variations in desaturase genes were observed; however, they did not alter the 3D structure of any desaturase protein and were not correlated with FA levels among the ten linseed varieties, which had different ALA contents. This suggests a complex regulatory process of biosynthesis of FAs in linseed. © 2016 Society of Chemical Industry