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Glucosinolate composition of Colombian accessions of mashua ( Tropaeolum tuberosum Ruíz & Pavón), structural elucidation of the predominant glucosinolate and assessment of its antifungal activity
Author(s) -
Martín Jenny Carolina,
Higuera Blanca Ligia
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7689
Subject(s) - glucosinolate , biology , rhizoctonia solani , phytophthora infestans , botany , brassicaceae , antifungal , horticulture , food science , blight , microbiology and biotechnology , brassica
BACKGROUND The content of individual and total glucosinolates in 65 mashua tuber accessions ( Tropaeolum tuberosum ) from the germplasm bank at Universidad Nacional de Colombia was determined by reverse phase high‐performance liquid chromatography on enzymatically desulfated extracts. The predominant glucosinolate was identified and the possible structure of the glucosinolate present in lower proportion was postulated from evidence obtained by high‐performance liquid chromatography/mass spectrometry, 1 H and 13 C nuclear magnetic resonance and bi‐dimensional experiments. The biological action of the hydrolysis products generated from the glucosinolates in the accessions that showed a higher content of these compounds was assessed in the presence of Fusarium oxysporum f. sp. dianthi , Rhizoctonia solani and Phytophthora infestans . RESULTS The total content of glucosinolates ranged between >3.00 × 10 −1 and 25.8 µmol g −1 dry matter. p ‐Methoxybenzyl glucosinolate was identified as the predominant glucosinolate in Colombian mashua accessions; besides, the possible presence of p ‐hydroxybenzyl glucosinolate was postulated. In vitro assays established an important fungal growth inhibition of the potato pathogen P. infestan s. CONCLUSION The biological action from p ‐methoxybenzyl glucosinolate and p ‐hydroxybenzyl glucosinolate found in Colombian mashua accessions depends on their concentration, with the Tt30 accession, characterized for showing the highest content of glucosinolates, being the most promising to control the assessed pathogens. © 2016 Society of Chemical Industry