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Anti‐inflammatory and antioxidant activity of polyphenolic extracts from Lactuca sativa (var. Maravilla de Verano ) under different farming methods
Author(s) -
Adesso Simona,
Pepe Giacomo,
Sommella Eduardo,
Manfra Michele,
Scopa Antonio,
Sofo Adriano,
Tenore Gian Carlo,
Russo Mariateresa,
Di Gaudio Francesca,
Autore Giuseppina,
Campiglia Pietro,
Marzocco Stefania
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7622
Subject(s) - polyphenol , nutraceutical , lactuca , antioxidant , quercetin , reactive oxygen species , anti inflammatory , food science , biology , oxidative stress , chemistry , botany , biochemistry , pharmacology
BACKGROUND Besides their nutritional value, vegetables are a source of health‐promoting compounds, such as polyphenols, and their content can be influenced by the particular farming method. In this study polyphenolic extracts from Lactuca sativa (var. Maravilla de verano ) plants cultivated with different farming methods were chemically characterised and tested in vitro and ex vivo inflammation models. RESULTS The tested extacts (250–2.5 µg mL −1 ) were able to reduce both the inflammatory and oxidative stress in LPS ‐stimulated J774A .1 murine monocyte macrophage cells, by lowering the release of nitric oxide ( NO ) and reactive oxygen species ( ROS ) and promoting nuclear translocation of nuclear factor (erythroid‐derived 2)‐like 2; (Nrf2) and nuclear factor‐ κB ( NF‐κB ). In this regard, quantitative profiles revealed different amounts of polyphenols, in particular quercetin levels were higher in plants under mineral fertilised treatment. Those extract showed an enhanced anti‐inflammatory and antioxidant activity. CONCLUSION Our data showed the anti‐inflammatory and antioxidant potential of Maravilla de Verano polyphenolic extracts. The effect of farming methods on polyphenolic levels was highlighted. The higher reduction of inflammatory mediators release in extracts from plants cultivated under mineral fertilisation treatment was correlated to the higher amount of quercetin. These results can be useful for both nutraceutical or agronomic purposes. © 2016 Society of Chemical Industry