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Detoxification and safety evaluation of aflatoxin B 1 in peanut oil using alkali refining
Author(s) -
Ji Ning,
Diao Enjie,
Li Xiangyang,
Zhang Zheng,
Dong Haizhou
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7592
Subject(s) - aflatoxin , refining (metallurgy) , peanut oil , detoxification (alternative medicine) , chemistry , food science , microbiology and biotechnology , environmental science , biology , raw material , medicine , organic chemistry , alternative medicine , pathology
BACKGROUND Aflatoxin B 1 ( AFB 1 ) is often detected in peanut oil, which comes from contaminated peanuts. AFB 1 in peanut oil seriously threatens the health of consumers. However, there are few methods to effectively remove AFB 1 in peanut oil. This study aimed to use an alkali‐refining method to degrade AFB 1 in peanut oil efficiently without increasing the equipment of oil and fat refining. RESULTS The optimum detoxifying conditions of AFB 1 in peanut oil with alkali refining were established using response surface methodology ( RSM ), and the safety of peanut oil after being refined with alkali was evaluated based on the Ames tests and HepG2 cell viability. The results showed that AFB 1 in peanut oil was decreased from 34.78 to 0.37 µg kg −1 (98.94% reduction) under the optimum detoxifying conditions, i.e. when the initial temperature of alkali refining was 43.51 °C, the amount of excess alkali was 0.30%, the content of alkali solution was 23.42% and the end temperature of alkali refining was 77.07 °C. The acid value and color of peanut oil refined by alkali were improved significantly, while the peroxide value was increased within an acceptable level. The safety of peanut oil contaminated by AFB 1 was improved significantly after being refined with alkali. CONCLUSION These results indicate that alkali refining is an effective method for removing AFB 1 in peanut oil. The optimum detoxifying conditions of AFB 1 in peanut oil with alkali refining could be used to guide the production of oil companies for ensuring food safety. © 2015 Society of Chemical Industry