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Hen egg white ovomacroglobulin promotes fibroblast migration via mediating cell adhesion and cytoskeleton
Author(s) -
Geng Fang,
Huang Xi,
Ma Meihu
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7498
Subject(s) - cell migration , extracellular matrix , microbiology and biotechnology , fibroblast , focal adhesion , cell growth , wound healing , cell adhesion , cell , viability assay , cancer cell , actin cytoskeleton , chemistry , biology , cytoskeleton , biochemistry , signal transduction , cancer , immunology , in vitro , genetics
BACKGROUND Hen egg white ovomacroglobulin ( OVM ) possesses a variety of bioactivities and could potentially be used as a pharmaceutical agent. It has been reported that OVM is involved in wound healing and cancer pathological processes, and previous results suggest that OVM plays a potential role in cell proliferation and migration; however, this has not yet been proven. In the present study, the effects of OVM on fibroblast proliferation and migration were evaluated. RESULTS Results of cell counting, cell viability, and cell cycle indicated that proliferation of fibroblasts was not altered by OVM treatment. However, scratch assays showed that OVM could promote the migration of 3 T6 mouse embryonic fibroblasts and human skin fibroblasts ( HSF ). Also, the adhesion of HSF to the collagen matrix was also enhanced by OVM treatment. RT‐qPCR and western blot analysis showed that β 1 ‐integrin, β ‐tubulin, and β ‐actin were up‐regulated while E‐cadherin was down‐regulated in OVM ‐treated HSF cells. The effect of OVM was silenced after forming a complex with trypsin, suggesting that the protease inhibitory ability of OVM is important for its effect on cell migration. CONCLUSION These results suggested that promotion of OVM on cell migration was achieved by enhancing cell adhesion to extracellular matrix, reducing intercellular aggregation, and strengthening cytoskeleton. The finding of the promotion effect of OVM on cell migration is important for understanding its role in wound healing and cancer pathological processes. © 2015 Society of Chemical Industry