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Authentication of the origin of sucrose‐based sugar products using quantitative natural abundance 13 C NMR
Author(s) -
Monakhova Yulia B,
Diehl Bernd WK
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7456
Subject(s) - sugar , sugar beet , sucrose , chemistry , principal component analysis , cane , food science , artificial intelligence , horticulture , computer science , biology
BACKGROUND Due to possible falsification of sugar cane products with cheaper alternative (sugar beet) on the market, a simple analytical methodology needs to be developed to control the authenticity of sugar products. RESULTS A direct 13 C nuclear magnetic resonance ( NMR ) method has been validated to differentiate between sucrose‐based sugar products produced from sugar beet ( C 3 plant) and sugar cane ( C 4 plant). The method is based on calculating relative 13 C content of the C1 , C2 , C5 , and the C1 , C4 , C5 , C6 positions of the glycosyl and fructosyl moieties of the sucrose molecule, respectively. NMR acquisition parameters and data processing have been optimized to reach a high level of intraday and interday precision (<0.2%). Good linearity ( R 2 = 0.93) was obtained for the beet sugar–cane sugar blends containing from 0 to 100 wt% of beet sugar. The method was applied to ten commercial sucrose‐based sugar products of different botanical origin. Principal component analysis (PCA) was applied to the relative peak areas for replicate measurements to visualize the difference between studied products. CONCLUSION The 13 C NMR method is a good alternative to complex isotope ratio mass spectrometry measurements for routine detection and semi‐quantification of adulteration of commercial cane sugar (C 4 plant) with less expensive beet sugar (C 3 plant). © 2015 Society of Chemical Industry