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The effect of ferrous‐chelating hairtail peptides on iron deficiency and intestinal flora in rats
Author(s) -
Lin HuiMin,
Deng ShangGui,
Huang SaiBo,
Li YingJie,
Song Ru
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7452
Subject(s) - ferrous , chelation , chemistry , ferrous sulphate , bioavailability , food science , biochemistry , iron deficiency , ferritin , hydrolysate , hydrolysis , medicine , pharmacology , biology , anemia , inorganic chemistry , organic chemistry
BACKGROUND Chelating agents, such as small peptides, can decrease free iron content and increase iron bioavailability. They may have promising therapeutic potential and may prevent the pro‐oxidant effects of low molecular weight iron. Hairtail is a species of fish that is rich in easily digestible proteins. We extended this strategy for iron delivery by using an enzymatic hydrolysate of hairtail as the chelating agent and found that the ferrous‐chelating hairtail peptides have anti‐anaemic activity in Sprague–Dawley rats with anaemia. RESULTS The anti‐anaemic activity of ferrous‐chelating hairtail peptides prepared by enzymatic hydrolysis of the hairtail and ferrous chelation was studied in rat models of iron deficiency anaemia. After the end of the 35 d experiment, we noted significant differences in haemoglobin, mean corpuscular volume, haemoglobin distribution width, and ferritin concentrations between those animals supplemented with ferrous‐chelating hairtail peptides and FeSO 4 and healthy animals. There were no negative side effects on the animals' growth or behaviour. There was no obvious inflammation in the intestinal mucosa lamina propria and no unbalance of intestinal flora. CONCLUSION The novel ferrous‐chelating hairtail peptides may be a suitable fortificant for improving iron‐deficiency status. Our findings demonstrated that this multi‐tracer technique has many applications in nutritional research. © 2015 Society of Chemical Industry