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Physico‐chemical properties, antioxidant activities and antihypertensive effects of walnut protein and its hydrolysate
Author(s) -
Wang Xiuming,
Chen Haixia,
Li Shuqin,
Zhou Jiangchao,
Xu Jiangtao
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7379
Subject(s) - hydrolysate , antioxidant , chemistry , hydrolysis , food science , trypsin , enzyme , enzymatic hydrolysis , angiotensin converting enzyme , biochemistry , blood pressure , medicine
Abstract BACKGROUND Some food proteins hydrolysates are found to possess multiple health effects. In this study, walnut protein ( WP ) was enzymatically hydrolysed by alcalase and trypsin under optimal conditions. The physico‐chemical properties, antioxidant and angiotensin‐converting enzyme ( ACE ) inhibitory activities of WP , alcalase‐generated walnut protein hydrolysate (AWPH) and trypsin‐generated walnut protein hydrolysate (TWPH) were comparatively studied. Stability properties of the walnut protein hydrolysate (WPH) and the antihypertensive activity in spontaneously hypertensive rats ( SHRs ) were also investigated. RESULTS The WPH showed higher physico‐chemical properties, antioxidant activities, ACE inhibitory activity and stability against thermal treatment and gastrointestinal digestion than WP . The results of antihypertensive effects in SHRs showed that the most potent decrease of AWPH and TWPH in the systolic blood pressure occurred at 4 h (−26 mmHg ) and 6 h (−30 mmHg ) after administration. The study indicated that the WPH could significantly decrease the systolic blood pressure ( P < 0.05). CONCLUSION The WPH exhibited high physico‐chemical properties, potent inhibitory activities and high stability. TWPH was more effective than AWPH in the detected properties. The results would be helpful for the comprehensive utilisation of the walnut resources. © 2015 Society of Chemical Industry