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Effects of Lactobacillus paracasei 01 fermented milk beverage on protection of intestinal epithelial cell in vitro
Author(s) -
Chen YenPo,
Hsu ChihAn,
Hung WeiTing,
Chen MingJu
Publication year - 2016
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7331
Subject(s) - lactobacillus paracasei , probiotic , lactobacillus , intestinal epithelium , chemistry , food science , fermentation , microbiology and biotechnology , barrier function , chemokine , skimmed milk , biology , epithelium , inflammation , immunology , bacteria , genetics
BACKGROUND Intestinal protection is an important function of probiotics. However, there is no evidence that the potential probiotic Lactobacillus paracasei 01 is able to improve intestinal function. In the present study, the protective effect and underlying mechanisms of L. paracasei 01 on intestinal epithelial cell in vitro were investigated. RESULTS A fermented milk beverage containing L. paracasei 01 at 3 × 10 8 CFU mL −1 was made from skim milk powder, glucose, sucrose and isomaltooligosaccharide. Results indicated that L. paracasei 01 fermented milk beverage could reduce macromolecule permeability caused by dextran sodium sulfate‐induced damage. Besides, 50 mL L −1 fermented milk beverage with live L. paracasei 01 in the cell medium could increase intestinal epithelial cell Caco‐2 growth, decrease lipopolysaccharide/tumor necrosis factor‐α ( TNF ‐α)/interferon‐γ ( IFN ‐γ)‐induced Caco‐2 cell death and chemokine CCL ‐20 production. The protecting mechanisms involve promoting intestinal epithelial cell growth and intestinal epithelial integrity to strengthen the intestinal barrier against chemical and inflammatory stimuli‐induced damage. Inhibition of inflammatory cytokines and chemokine such as IFN ‐γ, TNF ‐α and CCL ‐20 also contributes to the beneficial effects of this product on epithelial function. CONCLUSION Lactobacillus paracasei 01 fermented milk beverage may be a new functional food with intestinal protecting effects in vitro . © 2015 Society of Chemical Industry