Premium
Combination of selenium‐enriched green tea polysaccharides and Huo‐ji polysaccharides synergistically enhances antioxidant and immune activity in mice
Author(s) -
Yuan Chengfu,
Li Zhihong,
Peng Fan,
Xiao Fangxiang,
Ren Dongming,
Xue Hui,
Chen Tao,
Mushtaq Gohar,
Kamal Mohammad Amjad
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7287
Subject(s) - malondialdehyde , splenocyte , glutathione peroxidase , superoxide dismutase , gtp' , chemistry , antioxidant , oxidative stress , immune system , pharmacology , glutathione , polysaccharide , tumor necrosis factor alpha , biochemistry , immunology , biology , enzyme
BACKGROUND The aim of this study was to investigate the influence of a combination of selenium‐enriched green tea polysaccharides (Se‐ GTP ) and Huo‐ji polysaccharides ( HJP ) on the immune function and antioxidant activity in mice. RESULTS The results showed that the indices of spleen and thymus were markedly increased, and the activity of natural killer ( NK ) cell was promoted in mice treated with the combination of Se‐ GTP and HJP . The combined treatment of Se‐ GTP and HJP also reduced the content of tumour necrosis factor‐α ( TNF ‐α) and interleukin‐6 ( IL ‐6) in splenocytes. In addition, the activities of glutathione peroxidase ( GPx ) and superoxide dismutase ( SOD ) were remarkably enhanced, and malondialdehyde ( MDA ) levels were significantly reduced in mice treated with combination of Se‐ GTP and HJP . Furthermore, the combined treatment of Se‐ GTP and HJP increased nuclear factor erythroid 2‐related factor (Nrf2) expression at mRNA and protein levels in splenocytes. The effects of the combination treatment of Se‐ GTP and HJP in mice were stronger than with Se‐ GTP or HJP treatment alone. CONCLUSION Our study suggests that the combined administration of Se‐ GTP and HJP can synergistically improve immune function and decrease the oxidative stress by enhancing the mechanisms involved in the clearance of free radicals. © 2015 Society of Chemical Industry