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Physiological changes and sHSPs genes relative transcription in relation to the acquisition of seed germination during maturation of hybrid rice seed
Author(s) -
Zhu LiWei,
Cao DongDong,
Hu QiJuan,
Guan YaJing,
Hu WeiMin,
Nawaz Aamir,
Hu Jin
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7283
Subject(s) - apx , germination , sprouting , abscisic acid , oryza sativa , biology , catalase , sugar , horticulture , gibberellin , peroxidase , seedling , starch , botany , antioxidant , food science , gene , enzyme , biochemistry
BACKGROUND During the production of early hybrid rice seed, the seeds dehydrated slowly and retained high moisture levels when rainy weather lasted for a couple of days, and the rice seeds easily occurred pre‐harvest sprouting ( PHS ) along with high temperature. Therefore it is necessary to harvest the seeds before the PHS occurred. RESULTS The seeds of hybrid rice ( Oryza sativa L. subsp. indica ) cv. Qianyou No1 that harvests from 19 to 28 days after pollination ( DAP ) all had high seed vigour. The seed moisture content at 10 DAP was 36.1%, and declined to 28.6% at 19 DAP ; the contents of soluble sugar and total starch increased significantly with the development of seeds. The soluble protein content, the level of abscisic acid ( ABA ) and gibberellin ( GA 3 ), and ascorbate peroxidase ( APX ) activity continued to decrease from 10 DAP to 19 DAP . The seeds at 19 DAP had the highest peroxidase ( POD ) activity and lowest catalase ( CAT ) activity while the superoxide dismutase ( SOD ) activity had no significant difference among the different developing periods. The relative expressions of genes 64S Hsp18.0 and Os03g0267200 transcripts increased significantly from 10 to 19 DAP , and then decreased. However, no significant change was recorded in soluble protein, sugar and GA 3 after 16 DAP , and they all significantly correlated with seed viability and vigour during the process of seed maturity. CONCLUSION The seeds of hybrid rice Qianyou No1 had a higher viability and vigour when harvested from 19 DAP to 28 DAP , the transcription levels of 64S Hsp18.0 and Os03g0267200 increased significantly from 10 DAP to 19 DAP and the highest value was recorded at 19 DAP . The seeds could be harvested as early as 19 DAP without negative influence on seed vigour and viability. © 2015 Society of Chemical Industry