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(Anti)mutagenic and immunomodulatory properties of quercetin glycosides
Author(s) -
Valentová Kateřina,
Šíma Petr,
Rybková Zuzana,
Křížan Jiří,
Malachová Kateřina,
Křen Vladimír
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7251
Subject(s) - rutin , quercetin , in vivo , chemistry , immune system , flavonols , biochemistry , in vitro , biology , antioxidant , immunology , microbiology and biotechnology
BACKGROUND Quercetin‐3‐ O ‐β‐ d ‐glucopyranoside (isoquercitrin) and quercetin‐3‐ O ‐rutinoside (rutin) are common components of a normal human diet and are increasingly used in food supplements. Here their effect on mutagenesis and immunity is shown. RESULTS The in vitro (anti)mutagenic potential was compared with that of quercetin using the Ames test in Salmonella typhimurium His − strains TA100 , TA98 and TA102 . Isoquercitrin only slightly increased the number of revertants, while rutin was totally non‐mutagenic. On the other hand, all compounds displayed dose‐dependent protective activity against H 2 O 2 ‐ and tert ‐butyl hydroperoxide‐induced oxidative damage to the TA102 strain and at 75 µmol L −1 inhibited H 2 O 2 /Fe 2+ ‐induced formation of the open circular and linear forms of the DNA plasmid pBSIISK (−). In mice, none of the flavonols (0.86 µmol day −1 , 34 days) induced harmful effects. In immunized animals, all compounds enhanced ex vivo B cell proliferation; quercetin stimulated lymphocyte basal proliferation and increased the number of IgM ‐producing lymphocytes. Rutin promoted NK cytotoxic activity, supported T cells and enhanced gut epithelium renewal. No effect on IgG ‐forming cells was found. CONCLUSION Isoquercitrin displayed negligible and rutin no mutagenicity, but both showed significant antimutagenic and DNA ‐protective effects against oxidative damage. In vivo , they supported the readiness of the immune system for specific humoral immune response. © 2015 Society of Chemical Industry