Premium
Structural characteristics of pineapple pulp polysaccharides and their antitumor cell proliferation activities
Author(s) -
Wang Ling,
Tang DeQiang,
Kuang Yu,
Lin FengJiao,
Su Yu
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7185
Subject(s) - polysaccharide , chemistry , antioxidant , food science , glycosidic bond , monosaccharide , taste , functional food , biochemistry , enzyme
BACKGROUND Pineapple has a delicious taste and good health benefits. Bioactive polysaccharides are important components of pineapple that might contribute to its health benefits. Since little structural information on these polysaccharides is currently available, the aim of this study was to investigate their structural characteristics and bioactivities. RESULTS The polysaccharides of pineapple pulp were fractionated into three fractions ( PAPs 1–3) by anion exchange chromatography. Their structural characteristics were first identified, including molecular weights and glycosidic linkages. The monosaccharide compositions were revealed as PAP 1 (Ara, Xyl, Man, Glc and Gal), PAP 2 (Rha, Ara, Xyl, Man, Glc and Gal) and PAP 3 (Rha, Ara, Xyl, Man and Gal). Nuclear magnetic resonance ( NMR ) spectra suggested that PAP 2 had a backbone of → 4)‐ α ‐ d ‐Man p ‐(1 → 2,4)‐ α ‐ d ‐Man p ‐(1 → with branches attached to O‐4 of Man p . The NMR data of α ‐ l ‐Ara f ‐(1→, →3)‐ α ‐ l ‐Ara f ‐(1→, →4)‐ β ‐ d ‐Gal p ‐(1 → and → 4)‐ α ‐ d ‐Gal p AMe‐(1 → were assigned. PAPs 1 and 2 showed significant antitumor cell proliferation activities against breast carcinoma cell line and strong antioxidant activities. CONCLUSION The above findings indicated that PAPs 1–3 contributed much to the health benefits of pineapple. They could be used as health‐beneficial food additives in functional foods. © 2015 Society of Chemical Industry