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Rapid detection of ochratoxin A on membrane by dot immunogold filtration assay
Author(s) -
Chen Weifeng,
Jin Yucui,
Liu Aiping,
Wang Xiaohong,
Chen Fusheng
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7130
Subject(s) - ochratoxin a , citrinin , colloidal gold , chemistry , mycotoxin , aflatoxin , zearalenone , nitrocellulose , detection limit , chromatography , filtration (mathematics) , patulin , immunogold labelling , membrane , food science , biochemistry , nanotechnology , biology , nanoparticle , materials science , botany , statistics , mathematics , ultrastructure
BACKGROUND Ochratoxin A ( OTA ), a widely distributed mycotoxin produced by certain species of Aspergillus and Penicillium , has been identified as a carcinogenic, hepatotoxic, teratogenic, nephrotoxic and immunotoxic toxin. To reduce the risk of OTA contamination, a rapid, inexpensive, suitable and on‐site assay for its detection is required. RESULTS In this study a dot immunogold filtration assay ( DIGFA ) of OTA on high‐flow nitrocellulose membrane was developed. Firstly colloidal gold was synthesized and colloidal gold–polyclonal antibody ( PcAb ) conjugates against OTA were prepared at the optimal colloidal gold‐labeled pH value and package amount. Then the colloidal gold– PcAb conjugates were used to develop the OTA DIGFA . The results demonstrated a visual detection limit of approximately 10 ng mL −1 OTA . In addition, this method had no cross‐reaction with zearalenone, aflatoxin B1 or citrinin. CONCLUSION These results indicated that the developed DIGFA could be applied for the actual detection of samples without complicated steps. © 2015 Society of Chemical Industry