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Antioxidant activity and high‐performance liquid chromatographic analysis of phenolic compounds during in vitro callus culture of Plantago ovata Forsk. and effect of exogenous additives on accumulation of phenolic compounds
Author(s) -
Talukder Pratik,
Talapatra Shonima,
Ghoshal Nirmalya,
Sen Raychaudhuri Sarmistha
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7086
Subject(s) - callus , polyphenol , plantago ovata , antioxidant , chemistry , rutin , food science , gallic acid , biochemistry , botany , biology
Abstract BACKGROUND Plantago ovata , commonly called psyllium, is known to be a rich source of polyphenolic compounds. The present study was aimed at determining polyphenol content and studying their antioxidant activities in P. ovata during in vitro callus culture. An attempt was also made to enhance polyphenol content using external additives. The role of PAL gene in polyphenol accumulation was also studied. RESULTS The study indicated the presence of significant amounts of polyphenols, including flavonoids, in P. ovata callus. A gradual increase in polyphenol and flavonoid content was observed up to the third passage (63 days) of callus culture, which declined at the next passage. The third‐passage callus showed highest antioxidant activity. High‐performance liquid chromatographic results indicated the presence of high amounts of gallic acid and rutin in P. ovata calli; however, other polyphenols were also present but to a lesser extent. Additive supplementation was effective in enhancing polyphenol production and in increasing antioxidant activity in P. ovata callus. CONCLUSION The present research reported accumulation of polyphenols in callus culture of P. ovata , which could be applied to isolation of polyphenols for various beneficial purposes. It also indicated enhancement in the production of several polyphenols and also an increase in antioxidant activity in the additive‐treated callus. © 2015 Society of Chemical Industry