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Improving the catalytic behavior of inulin fructotransferase under high hydrostatic pressure
Author(s) -
Li Yungao,
Miao Ming,
Chen Xiangyin,
Jiang Bo,
Liu Miao,
Feng Biao
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.7071
Subject(s) - chemistry , hydrostatic pressure , inulin , bioconversion , catalysis , volume (thermodynamics) , enzyme , reaction conditions , chemical engineering , food science , fermentation , biochemistry , thermodynamics , physics , engineering
BACKGROUND The demand for difructose anhydride III ( DFA III ), a novel functional sweetener, is growing continuously. It is produced from inulin by inulin fructotransferase ( IFTase ). In this study, high hydrostatic pressure ( HHP ), as a clean technology, was first applied to further improve the catalytic efficiency of IFTase in the process. RESULTS The maximum activity of IFTase was obtained under 200 MPa at 60 °C. Meanwhile, HHP lowered the energy barrier necessary for the enzymatic reaction and decreased the volume between the reactants and the transition state. Under this condition, the optimal pH for the enzymatic reaction shifted from 5.5 to 6.0. The activity was further enhanced by 65.2% in the presence of 1.5 mol L −1 NaCl . CONCLUSION The catalytic reaction of IFTase was performed under HHP for the first time. HHP , as a promising green technology for bioconversion, significantly accelerated the enzymatic reaction under the appropriate operational conditions. © 2015 Society of Chemical Industry