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Infrared–metabolomics approach in detecting changes in Andrographis paniculata metabolites due to different harvesting ages and times
Author(s) -
Yusof Nur A'thifah,
Isha Azizul,
Ismail Intan Safinar,
Khatib Alfi,
Shaari Khozirah,
Abas Faridah,
Rukayadi Yaya
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6987
Subject(s) - andrographis paniculata , andrographolide , metabolite , chemistry , metabolomics , chemometrics , fourier transform infrared spectroscopy , morning , principal component analysis , botany , biology , chromatography , mathematics , biochemistry , medicine , physics , alternative medicine , statistics , pathology , quantum mechanics
BACKGROUND The metabolite changes in three germplasm accessions of Malaysia Andrographis paniculata (Burm. F.) Nees, viz. 11265 (H), 11341 (P) and 11248 (T), due to their different harvesting ages and times were successfully evaluated by attenuated total reflectance ( ATR )–Fourier transform infrared ( FTIR ) spectroscopy and translated through multivariate data analysis of principal component analysis ( PCA ) and orthogonal partial least square–discriminant analysis ( OPLS‐DA ). This present study revealed the feasibility of ATR‐FTIR in detecting the trend changes of the major metabolites – andrographolide and neoandrographolide – functional groups in A. paniculata leaves of different accessions. The harvesting parameter was set at three different ages of 120, 150 and 180 days after transplanting ( DAT ) and at two different time sessions of morning (7:30–10:30 am) and evening (2:30–5.30 pm). RESULTS OPLS‐DA successfully discriminated the A. paniculata crude extracts into groups of which the main constituents – andrographolide and neoandrographolide – could be mainly observed in the morning session of 120 DAT for P and T, while H gave the highest intensities of these constituents at 150 DAT . CONCLUSION The information extracted from ATR‐FTIR data through OPLS‐DA could be useful in tailoring this plant harvest stage in relation to the content of its two major diterpene lactones: andrographolide and neoandrographolide. © 2014 Society of Chemical Industry