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Bioactives in cactus ( Opuntia ficus‐indica ) stems possess potent antioxidant and pro‐apoptotic activities through COX ‐2 involvement
Author(s) -
Kim Jinhee,
Soh Soon Yil,
Shin Juha,
Cho ChiWoung,
Choi Young Hee,
Nam SangYong
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6968
Subject(s) - abts , chemistry , dpph , polyphenol , ethyl acetate , apoptosis , antioxidant , traditional medicine , biochemistry , cytotoxicity , cactus , cancer cell , moraceae , in vitro , biology , botany , cancer , medicine , genetics
BACKGROUND Bioactives extracted from cactus ( Opuntia ficus‐indica ) stems were investigated for their chemopreventive activities using human cancer cells in vitro . The bioactives present in crude extracts were detected and quantified using high‐performance liquid chromatography. RESULTS Among all the extracts, such as hexane, ethyl acetate ( EtOAc ), acetone, methanol ( MeOH ), and MeOH :water (80:20), the MeOH extract had the highest amount of polyphenolic compounds and the acetone extract exhibited the most potent effect at scavenging the 2,2,‐diphenyl‐1‐picrylhydrazyl ( DPPH ) and 2,2′‐azino‐di‐(3‐ethylbenzothiazoline)‐6‐sulfonic acid ( ABTS •+ ) radical. In addition, most of the extracts, with the exception of hexane, exhibited significant cytotoxicity in human SW480 colon and MCF7 breast cancer cells. Overall, the SW480 cells were more sensitive than the MCF7 cells to the cytotoxic effect of the O. ficus‐indica extracts ( OFEs ). Cell death by OFE treatment caused significant inhibition of cyclooxygenase‐2 and increased the Bax/Bcl2 ratio in both SW480 and MCF7 cell lines. However, degradation of poly ( ADP ‐ribose) polymerase was significantly increased by OFE only in the MCF7 cells, thereby inducing apoptosis. CONCLUSION These findings demonstrate the health‐benefit roles, including anti‐oxidative and anti‐proliferative activities as well as pro‐apoptotic effects, of bioactive compounds in OFEs , suggesting a chemopreventive role in human cancer cells. © 2014 Society of Chemical Industry