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Dispersive liquid–liquid microextraction combined with non‐aqueous capillary electrophoresis for the determination of imazalil, prochloraz and thiabendazole in apples, cherry tomatoes and grape juice
Author(s) -
Xu Lina,
Luan Feng,
Liu Huitao,
Gao Yuan
Publication year - 2014
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6834
Subject(s) - chromatography , capillary electrophoresis , chemistry , fruit juice , aqueous solution , aqueous extract , food science , traditional medicine , organic chemistry , medicine
BACKGROUND Fruit and vegetables are frequently treated with fungicides to reduce possible spoilage. As a result, fungicide residues may be accumulated in derived products. This important group of chemical compounds has been heavily regulated because of their potential toxicity. Therefore, a simple and rapid method to determine fungicides is desired. RESULTS A simple non‐aqueous capillary electrophoresis (NACE) method based on dispersive liquid–liquid microextraction (DLLME) has been proposed for the determination of imazalil, prochloraz and thiabendazole fungicides in fruits and juice samples. Separation buffer consisted of a methanol–acetonitrile mixture (35:65, v/v) containing 30 mmol L −1 ammonium chloride and 0.5% phosphoric acid. The optimum DLLME conditions were 80 µL trichloromethane as extraction solvent, 0.5 mL tetrahydrofuran as disperser solvent, sample solution pH at 6.0, 5% (w/v) NaCl and 10 s extraction time. Recoveries obtained for various samples ranged from 72% to 102%, with relative standard deviation lower than 6.4%. The limits of detection ranged from 0.47 to 0.72 µg kg −1 . CONCLUSION The proposed method takes the advantages of DLLME and NACE. It is rapid, accurate, sensitive and reproducible for the determination of imazalil, prochloraz and thiabendazole in fruit samples. © 2014 Society of Chemical Industry