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Molecular diversity and hypoglycemic polypeptide‐P content of Momordica charantia in different accessions and different seasons
Author(s) -
Tian Miao,
Zeng XiangQing,
Song HuanLei,
Hu ShanXin,
Wang FuJun,
Zhao Jian,
Hu ZhiBi
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6826
Subject(s) - momordica , biology , diversity (politics) , food science , botany , traditional medicine , medicine , anthropology , sociology
BACKGROUND Momordica charantia (MC) has been used for treating diabetes mellitus from ancient times in Asia, Africa and South America. There are many MC accessions in local markets. Polypeptide‐P as a main hypoglycemic component in MC was first studied in this experiment to illustrate the different contents in MC of different accessions and different harvesting times. RESULTS Nineteen MC accessions collected from different regions were clustered into three groups using random amplified polymorphic DNA (RAPD) and inter‐simple sequence repeat (ISSR) molecular markers. Content of polypeptide‐P in the tested MC accessions was detected by western blot (WB) method. The WB results revealed that polypeptide‐P was detected in MC accessions harvested in June and July but not in September and October. Furthermore, Polypeptide‐P content corresponded well with the MC accessions. CONCLUSION Our results suggest that the MC accessions and the harvesting times or the weather during harvest play significant roles in high content of polypeptide‐P. [[ArtCopyrightmsg]]