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Characterization of Phytophthora nicotianae isolates in southeast Spain and their detection and quantification through a real‐time TaqMan PCR
Author(s) -
Blaya Josefa,
Lacasa Carmen,
Lacasa Alfredo,
Martínez Victoriano,
SantísimaTrinidad Ana B,
Pascual Jose A,
Ros Margarita
Publication year - 2015
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.6813
Subject(s) - phytophthora nicotianae , biology , pepper , internal transcribed spacer , phytophthora capsici , taqman , ribosomal dna , phytophthora , botany , ribosomal rna , genetics , horticulture , polymerase chain reaction , gene , phylogenetics
BACKGROUND The soil‐borne pathogens Phytophthora nicotianae and P. capsici are the causal agents of root and stem rot of many plant species. Although P. capsici was considered the causal agent in one of the main pepper production areas of Spain to date, evidence of the presence of P. nicotianae was found. We aimed to survey the presence of P. nicotianae and study the variability in its populations in this area in order to improve the management of Tristeza disease. RESULTS A new specific primer and a TaqMan probe were designed based on the internal transcribed spacer regions of ribosomal DNA to detect and quantify P. nicotianae . Both morphological and molecular analysis showed its presence and confirmed it to be the causal agent of the Phytophthora disease symptoms in the studied area. The genetic characterization among P. nicotianae populations showed a low variability of genetic diversity among the isolates. Only isolates of the A2 mating type were detected. CONCLUSIONS Not only is a specific and early detection of P. nicotianae essential but also the study of genetic variability among isolates for the appropriate management of the disease, above all, in producing areas with favorable conditions for the advance of the disease. © 2014 Society of Chemical Industry